Table of Contents: Articles

Increased Process Understanding Through Monitoring and Scale-Down Models: Case Study of a Cell Culture Harvest Fluid Titration and Filtration Process
by Kurt Yanagimachi, Corey Dodge, and Marisa Hewitt
This article presents a case study demonstrating the use of detailed process monitoring and scale-down modeling in determining the root causes of yield losses in the manufacture of a therapeutic enzyme and identifying alternative technologies to improve the manufacturing process.
Case Study: Parenteral Facility Upgrade Project with Fill Line Install
by Keith Weseli and Michael DiGiovanni
This article presents a case study illustrating project management and commissioning and qualification processes that allowed for accelerated completion of a renovation project.
Integrating Industrial Engineering and Lean Techniques at a Contract Pharmaceutical Manufacturer
by Valerie Maier-Speredelozzi, Cyrus Agarabi, Thomas Needham, and Sirine A. Saleem
This article presents the application of industrial engineering and lean techniques to a contract pharmaceutical manufacturing facility.
Retrofitting CIP into API Plants
by Nigel A. Fletcher
This article describes the physical modifications and additions retrofitted into existing plants to incorporate CIP technology and some of the techniques that can be used to ‘stretch’ the existing CIP systems for best effect.
Solving the Terminology Conundrum
by Robert Adamson, Nuala Calnan, Robert E. Chew, and Steven J. Wisniewski
This article discusses the terms “commissioning,” “qualification,” and “verification.” Do the terms refer to the same or different ideas? How should the pharmaceutical and biotechnology industries use these terms in a consistent and meaningful way? This article provides a compilation of how these terms are used in regulations and by various industries, and provides a proposal for clear definitions to be used as ISPE updates and creates Baseline® Guides.

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May 2011 Volume 43 Number 5, pp 389 - 499

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Nature Reviews Genetics
Focus on Gene-Based Therapies
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EDITORIAL
----------------------
No second thoughts about data access p389
doi:10.1038/ng.827
More data than we can handle is no excuse to give up our efforts to
promote data access, but it may make us think about new ways to make it sustainable.
http://links.ealert.nature.com/ctt?kn=74&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0

Variants modulating the expression of a chromosome domain encompassing PLAG1
influence bovine stature pp405 - 413
Latifa Karim et al.
doi:10.1038/ng.814
Michel Georges and colleagues report fine mapping and functional characterization
of a quantitative trait locus with a large effect on bovine stature. They show
that the likely causal variants at this locus modulate expression of a
chromosomal domain encompassing PLAG1 and several other genes.
Abstract: http://links.ealert.nature.com/ctt?kn=32&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=91&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0

Variants modulating the expression of a chromosome domain encompassing PLAG1
influence bovine stature pp405 - 413
Latifa Karim et al.
doi:10.1038/ng.814
Michel Georges and colleagues report fine mapping and functional characterization
of a quantitative trait locus with a large effect on bovine stature. They show
that the likely causal variants at this locus modulate expression of a
chromosomal domain encompassing PLAG1 and several other genes.
Abstract: http://links.ealert.nature.com/ctt?kn=32&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=91&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0

Glycerol-3-phosphate is a critical mobile inducer of systemic immunity in plants pp421 - 427
Bidisha Chanda et al.
doi:10.1038/ng.798
Pradeep Kachroo and colleagues show that glycerol-3-phosphate is a critical mobile
inducer of systemic acquired resistance in plants.
Abstract: http://links.ealert.nature.com/ctt?kn=25&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=104&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0


Mutant nucleophosmin and cooperating pathways drive leukemia initiation
and progression in mice pp470 - 475
George S Vassiliou et al.
doi:10.1038/ng.796
George Vassiliou, Allan Bradley and colleagues perform a Sleeping Beauty
transposon mutagenesis screen to identify insertions that cooperate with
mutant Npm1 to produce acute myeloid leukemia (AML) in mice. They observed
mutually exclusive integrations in Csf2, Flt3 or Rasgrp1 in a high percentage
of cases, providing insights into the molecular pathogenesis of this AML subtype.
Abstract: http://links.ealert.nature.com/ctt?kn=124&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=53&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0

A framework for variation discovery and genotyping using next-generation
DNA sequencing data pp491 - 498
Mark A DePristo et al.
doi:10.1038/ng.806
Mark DePristo and colleagues report an analytical framework to discover
and genotype variation using whole exome and genome resequencing data
from next-generation sequencing technologies. They apply these methods
to low-pass population sequencing data from the 1000 Genomes Project.
Abstract: http://links.ealert.nature.com/ctt?kn=127&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0
Article: http://links.ealert.nature.com/ctt?kn=5&m=36557924&r=MzU0NzkwNjQwNAS2&b=2&j=MTAwMTY2NDYzS0&mt=1&rt=0

MCQs in Pharmaceutical Calculations

MCQs in Pharmaceutical Calculations

This is a revision guide in calculations for pharmacy students and registration trainees. This text is part of the RPS "Tomorrow's Pharmacist" series. The

The rapid progress in biology will continue to change the methodological approach to drug discovery in the coming years. Electronic media will continuously help researchers to access and share information. It is, however, becoming more and more evident that many young pharmacologists have only limited training in classical pharmacological methodologies. These long-standing and still highly relevant methods are simply not available in the electronic databases. Drug Discovery and Evaluation:Pharmacological Assays bridges this gap by comprehensively covering the pharmacological methods that have been utilized successfully for more than a hundred years as well as the latest technologies.

The classical methods and the newest technologies, all in one book!

The 3rd edition of this successful reference book contains an updated selection of the most frequently used assays for reliably detecting the pharmacological effects of potential drugs. Effects covered include cardiovascular, analgesic, endocrine, psychotropic, respiratory, renal, and immunomodulatory activities. Each of the more than 1000 assays comprises a detailed protocol outlining the purpose and rationale of the method, a critical assessment of the results and their pharmacological and clinical relevance. In addition, animal models of rare diseases are described.

For this third edition, all existing chapters have been revised and completely updated. A large number of assays were added: we are now publishing two volumes. Sections that have been specifically enlarged include - Pharmacological assays in thrombosis and haemostasis (formerly called activity of blood constituents), - Antidiabetic activity (includes completely new chapters such as Biochemical Methods in Diabetology), - Anti-atherosclerotic activity.

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Cocaine (Drugs: the Straight Facts) (US$30)
Stationary Office Pages: 112 2003-03 ISBN: 0791072606 10 MB
Cocaine is a very addictive drug. Cocaine is a stimulant it directly affects your brain. Cocaine stimulates certain nerve cells in the brain, producing feelings of intense pleasure. Users talk about feeling carefree, or relaxed, or utterly in control. But this artificial stimulation comes with a price tag. The “high” from cocaine lasts only from five to 20 minutes, and you will need more and more cocaine each time you try to match the feelings of that first, initial experience. Cocaine causes severe mood swings and irritability. As soon as cocaine enters your bloodstream it goes to work, increasing your heart rate and raising your blood pressure. It increases your body’s temperature and causes the pupils of your eyes to dilate. Repeated sniffing of cocaine powder causes your nose to become irritated and frequently runny. The cocaine can even eat away at the cartilage in your nose, producing holes.

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This revised edition assesses the range of non-prescription, over-the-counter medicines that can be recommended for the most frequently encountered minor illnesses. The first edition has been updated to reflect changes in the legal status and availability of products and new opinions on and approaches to treatment. A new section on emergency hormonal contraception is also included. The information is presented in a structured formulary format with products described both generically and by brand name along with supplier details. Each chapter also contains a summary of key points with suggestions regarding the most appropriate products. The book should enable pharmacists, GPs, nurses and other healthcare professionals to make well-informed recommendations and to give patients and customers sound advice on non-prescription medicines.

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